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. 2018 Oct 4;33(11):2060–2073. doi: 10.1093/humrep/dey290

Figure 7.

Figure 7

AQP3 influenced reorganization of the actin cytoskeleton and ezrin. (A) Organization of the actin cytoskeleton in RL95-2 cells treated with or without AQP3 siRNA, respectively (a, b). E2P4 induced the reorganization of the actin cytoskeleton by promoting filopodia formation and stress fiber rearrangement (d). Treating RL95-2 cells with AQP3 siRNA (c) significantly reduced E2P4-regulated reorganization of the actin cytoskeleton. AQP3 overexpression influenced reorganization of the actin cytoskeleton (f). The images are magnified ×1000. Bar = 5 μm. White arrows indicate filopodia; red arrows indicate lamellipodia. Experiments were repeated four times. (B) Ezrin organization in RL95-2 cells treated with or without AQP3 siRNA, respectively (a, b). E2P4 induced ezrin reorganization by promoting ezrin expression (d). Treating RL95-2 cells with AQP3 siRNA (c) significantly reduced E2P4-regulated ezrin reorganization but not its expression. The images are magnified ×630. Bar = 15 μm. White arrows indicate ezrin. Experiments were repeated four times. (C) AQP3 influenced the expression of EMT-related factors. Knockdown of AQP3 in RL95-2 cells inhibited the E2P4-induced EMT (a–d). The data are presented as the means ± SD, *P < 0.05 and **P < 0.01 (Student’s t-test). Experiments were repeated six times. E2P4 concentration: 10 nM estradiol and 1 μM progesterone.