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. Author manuscript; available in PMC: 2019 Nov 1.
Published in final edited form as: Breast Cancer Res Treat. 2018 Jul 28;172(1):69–82. doi: 10.1007/s10549-018-4884-x

Figure 3. Blockade of CD47 enhances macrophage mediated clearance of breast cancer cells.

Figure 3.

(A) Macrophage mediated cytotoxicity was measured in 16-well plates equipped with electrodes to measure cell impedance, cell cytotoxicity was counted as changes in cells index (impedance) when cells migrated to bottom plate equipped with electrodes N=4 *p<0.05 (B ) cell viability at 15h and (C) 24 h post treatment (*p<0.01). (D) Tumor tissue sections where stained with a fluorescent antibody to CD68 (FITC-green) to detect tissue infiltrating macrophages and DAPI to denote nuclei (blue), (E) sections were quantified by examining fluorescence of CD68/DAPI over area of section at 20x under multispectral fluorescent imaging (Scale bar = 50 μm). (F) HMGB1 expression was assessed by RT-PCR in tumor extracts (*p<0.05), (G) calreticulin gene expression was assessed in lysates from in vivo tumor studies by Western Blot hybridization and (H) was quantified by densitometry (*p<0.05).