Table 1.
Top 10 proteins identified from Csd5-VSV-G immunoprecipitation and mass spectrometry.
| Ranka |
Protein Name |
H. pylori G27 Locus Tag |
% Coverage (± SD )b |
AVG PSM (± SD)b,c |
Mass (kD) | Description |
|---|---|---|---|---|---|---|
| 1 | MurF | HPG27_696 | 64 ± 1 | 122 ± 7 | 56 | Cytoplasmic PG precursor synthase |
| 2 | AtpA | HPG27_1079 | 62 ± 1 | 85 ± 9 | 55 | α subunit: F1ATP Synthase |
| 3 | AtpD | HPG27_1077 | 76 ± 5 | 78 ± 3 | 51 | β subunit: F1ATP Synthase |
| 4 | Csd5 | HPG27_1195 | 52 ± 3 | 60 ± 6 | 22 | Cell shape protein |
| 5 | AtpG | HPG27_1078 | 52 ± 3 | 28 ± 2 | 34 | λ subunit: FOATP Synthase |
| 6 | CcmA | HPG27_1480 | 67 ± 9 | 27 ± 1 | 15 | Cell shape protein; Putative bactofilin |
| 7 | AtpH | HPG27_1080 | 57 ± 5 | 19 ± 3 | 20 | δ subunit: F1ATP Synthase |
| 8 | AtpF | HPG27_1081 | 34 ± 1 | 14 ± 1 | 20 | b subunit: FOATP Synthase |
| 9 | AtpC | HPG27_1076 | 53 ± 5 | 14 ± 2 | 13 | ε subunit: F1ATP Synthase |
| 10 | AtpX | HPG27_1082 | 49 ± 0 | 12 ± 1 | 16 | b’ subunit: FOATP Synthase |
a
Uniquely present in Csd5-VSV-G IP vs. LSH100 (no tag) negative control, except AtpD.
b
Average (AVG) and standard deviation (SD) from 3 technical replicates (3 injections per sample).
c
vs. 0 positive spectral matches (PSM) in LSH100 (except AtpD with 2 ± 2).