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. Author manuscript; available in PMC: 2019 Oct 5.
Published in final edited form as: Mol Microbiol. 2018 Oct 5;110(2):219–238. doi: 10.1111/mmi.14099

Fig. 6: Role on HfiA in Fla- holdfast misregulation.

Fig. 6:

(A) Quantification of cells harboring a holdfast in mixed populations grown to exponential phase. The results represent the average of at least two independent replicates (more than 250 cells per replicate) and the error bars represent the SEM. Statistical comparisons are calculated using Student’s unpaired t-tests. ** P < 0.01; * P < 0.1; ns = not significant. (B) ß-galactosidase activity of PhfiA-lacZ transcriptional fusions in PYE (left) and M2G (right). The results represent the average of at least 6 independent cultures (assayed on at least 2 different days) and the error bars represent the SEM. Statistical comparisons are calculated using Student’s unpaired t-tests. ** P < 0.01; * P < 0.1; ns = not significant. (C) Proposed model. Defects in flagellum synthesis result in an increase of c-di-GMP, which reduces transcription of the holdfast synthesis inhibitor gene hfiA, thereby resulting in an increase in holdfast synthesis.