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. Author manuscript; available in PMC: 2018 Nov 10.
Published in final edited form as: Oncogene. 2018 May 10;37(33):4534–4545. doi: 10.1038/s41388-018-0282-4

Figure 2. Activation of intracellular signaling pathways is involved in HERV-K transactivation by KSHV.

Figure 2

(A-B) HUVEC were transfected with control vector (pc) or vectors encoding LANA (pcLANA) or vFLIP (pcvFLIP) at 0.2, 1.0 or 2.5 μg, respectively, for 48 h, then protein expression was analyzed by using immunoblots. (C-F) HUVEC were first incubated with either vehicle or MEK inhibitor (10 μM of U0126) or NF-κB inhibitor (10 μM of Bay11-7082) for 1 h, then transfected or infected as described above. The induction of HERV-K transactivation was quantified by using qRT-PCR and protein expression was detected by immunoblots. Error bars represent the S.D. from 3 independent experiments. ** = p<0.01.