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. Author manuscript; available in PMC: 2018 Nov 10.
Published in final edited form as: Oncogene. 2018 May 10;37(33):4534–4545. doi: 10.1038/s41388-018-0282-4

Figure 5. The HERV-K NP9 protein is involved in the pathogenesis of KSHV long-term-infected endothelial cells.

Figure 5

(A) The protein expression in KSHV long-term-infected telomerase-immortalized human umbilical vein endothelial (TIVE-LTC) and parental uninfected TIVE cells was detected and compared by immunoblots. (B) Immunoprecipitation assays were performed with anti-LANA antibody as described previously. (C-E) The stably “knock-down” of Np9 in TIVE-LTC were established by using lentiviral vector containing shRNA specifically targeting Np9 (Np9-shRNA) as described in the Methods. A non-silencing (n)-shRNA was used as a negative control. The protein expression, cell invasiveness and anchorage-independent growth abilities were measured as described above.