Figure 4. RsBluE and RcBluE restore AdoCbl synthesis in a S. enterica ΔpduX strain.

Growth analysis of S. enterica pduX⁺ and ΔpduX strains harboring plasmids expressing bluE⁺ from R. sphaeroides (R.s. bluE⁺), R. capsulatus (R.c. bluE⁺), PduX from S. enterica (S.e. pduX⁺), or containing the empty vector pBAD24 (vector). Cells grown aerobically at 37°C in NCE minimal medium with ethanolamine (90 mM) as the sole carbon and energy source, supplemented with DMB (0.15 mM), arabinose (0.5 mM), ampicillin (0.1 mg mL⁻¹), MgSO₄ (1 mM), Fe(III)-citrate (0.05 mM), and A. Cby (300 nM) or B. Cbi (300 nM). A representative graph of three independent growth experiments performed in technical triplicate. Error bar represent the standard error of the mean. Figure key: ΔpduX/pRsBluE (□), ΔpduX/pSePduX (▲), ΔpduX/pRcBluE (■), ΔpduX/vector (○), pduX⁺/pSePduX (●), pduX⁺/vector (△).