Table 1. Comparison of RsBluE and SePduX activities as a function of substrates.

Specific activity values for purified and sarkosyl solubilized RsBluE and SePduX enzymes were assayed for ATPase activity in the presence and absence of L-Thr or L-Ser. Values are reported as mean ± standard deviation of three measurements of activity at 100 mM ATP and 50 mM L-Thr or L-Ser. Activity was measured with a NADH consumption assay (see Materials and Methods).

Protein	ATP (μmol ATP min−1 mg−1)	L-Thr (μmol ATP min−1 mg−1)	L-Ser (μmol ATP min−1 mg−1)
RsBluE	0.93 ± 0.02	0.77 ± 0.02	0.42 ± 0.01
SePduX	1.00 ± 0.03	0.91 ± 0.03	0.65 ± 0.02