Figure 3.

ΔCTL protofilaments form extended bright structures in addition to dynamic clusters. A. Contrast enhanced TIRFM images of structures observed on 20% DOPG 80% DOPC SLBs for FtsZ or CTL variants flowed in with 2 mM GTP at steady state or during flow. The FtsZ variants in each of the flow cells are 2 μM FtsZ or CTL variant (6% FtsZ-Alexa488 or corresponding Alexa488-labeled CTL variant) and 2 μM C-terminal MTS fusions replacing the CTC of FtsZ or corresponding CTL variant. B. Representative contrast enhanced TIRFM images showing the disassembly of an extended bright structure formed by ΔCTL/ΔCTL-MTS on the membrane over time. Scale bar – 10 μm. Reaction buffer contains 50 mM HEPES pH 8.0, 0.1 mM EDTA, 10 mM MgCl2, 300 mM KCl, 1% Glycerol, 0.1 mg mL⁻¹ Casein (blocking agent), 0.5 mg mL⁻¹ ascorbate. Structures presented are representative and were confirmed using at least 3 independent replicates. Extended bright structures were observed in all replicates for ΔCTL/ΔCTL-MTS.