Table 2.
Oligonucleotide primers for multiplex toxin PCR
| Gene | Primers | Sequence (5′-3′) | size | Conc.* | Reference |
|---|---|---|---|---|---|
| netB | JRP6656 JRP6655 |
CTTCTAGTGATACCGCTTCAC CGTTATATTCACTTGTTGACGAAAG |
738 bp | 0.6 0.6 |
V. Adams & J. Rood |
| etx | JRP4234 JRP4235 |
CCACTTACTTGTCCTACTAAC GCGGTGATATCCATCTATTC |
656 bp | 0.44 0.44 |
[78] |
| iap | JRP5507 JRP5508 |
GGAAAAGAAAATTATAGTGATTGG CCTGCATAACCTGGAATGGC |
461 bp | 0.5 0.5 |
V. Adams & J. Rood |
| plc | JRP4232 JRP4233 |
GCTAATGTTACTGCCGTTGACC CCTCTGATACATCGTGTAAG |
324 bp | 0.4 0.4 |
Based on [78] |
| cpe | JRP5179 JRP5180 |
GGAGATGGTTGGATATTAGG GGACCAGCAGTTGTAGATA |
233 bp | 0.3 0.3 |
[78] |
| cpb | JRP5181 JRP5182 |
GCGAATATGCTGAATCATCTA GCAGGAACATTAGTATATCTTC |
196 bp | 0.3 0.3 |
[78] |
*
Concentration of oligonucleotides in final reaction (μM)