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Skin irritation test of methylisothiazolinone (MIT) solubilized in Dulbecco’s Modified Eagle Medium (DMEM) using the EpiDerm™ 3D human skin model. EpiDerm™ 3D human skin models were treated with MIT solubilized in DMEM for 1 hr, and the cell viability of the skin model was measured by MTT assay. The control group was treated with only PBS without MIT, and 5% SDS was used as the positive chemical.
