Fig. 7.

Released IL-1α in the culture medium of EpiDerm™ treated with methylisothiazolinone (MIT) alone or its mixture with propylene glycol (PG). At 24 hr post-treatment, a small amount of the media was extracted and the IL-1α level was measured. In the positive control group treated with 5% SDS, a high level of IL-1α was detected. IL-1α was not induced by 0.01% MIT. However, it was increased by the mixture of PG and MIT (p < 0.01). A, 0.01% MIT in DMEM; B, 0.01% MIT in PG; C, 1% MIT in DMEM; D, 1% MIT in PG. **p < 0.01.