Table 1.

Bacteria and plasmids used in this study

Strain	Description	Source
E. coli MC1061 F′	Plasmid‐free, recA+, nonamber suppressor cloning strain	Lucigen
E. coli Nissle 1917	Nonpathogenic human commensal used in the probiotic, Mutaflor	University of Minnesota
E. coli Nissle 1917 RN	Rifampicin and nalidixic acid resistant derivative of E. coli Nissle 1917	University of Minnesota
E. faecium 8E9	Ampicillin/vancomycin/linezolid resistant	University of Minnesota
E. faecium 6E6	Ampicillin/vancomycin/linezolid resistant	University of Minnesota
E. faecium 6E6 ManPTS mutant	E. faecium 6E6 derivative with a basepair deletion in ManC (mutant A4)	49
E. faecalis V583R	Rifampicin resistant derivative of E. faecalis V583	Medical College of Wisconsin

Plasmid	Description	Source
pHK22	pACYC184 derivative containing 9.4 kb MicV production fragment	University of Minnesota
pBFFusion	E. coli vector with synthetic promoter, ProTeOn+ upstream of green fluorescent protein (GFP)	University of Minnesota
pBFVIcolVsec	pBFFusionΔGFP replaced with Microcin V and containing a 3.6 kb MicV secretion system fragment from pHK22	University of Minnesota
pMPES2:CbnA	pBFVIcolVsecΔMicV replaced with Carnobacteriocin gblock with a modular molecular cloning site	This study
pMPES2	pMPES2:CbnA lacking CbnA (only molecular cloning site)	This study
pMPES2:A	pMPES2 with Enterocin A	This study
pMPES2:B	pMPES2 with Enterocin B	This study
pMPES2:H	pMPES2 with Hiracin JM79	This study
pMPES2:HAB	pMPES2 with operon of Hiracin JM79:Enterocin A: Enterocin B	This study
pMPES2:BHA	pMPES2 with operon of Enterocin B:Hiracin JM79: Enterocin A	This study
pMPES2:ABH	pMPES2 with operon of Enterocin A:Enterocin B: Hiracin JM79	This study
pMPES2:HBA	pMPES2 with operon of Hiracin JM79:Enterocin B: Enterocin A	This study
pMPES2:BAH	pMPES2 with operon of Enterocin B:Enterocin A: Hiracin JM79	This study
pMPES2:AHB	pMPES2 with operon of Enterocin A:Hiracin JM79: Enterocin B	This study