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. 2018 Oct 20;24:28. doi: 10.1186/s40409-018-0167-6

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© The Author(s). 2018

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Isolation profile of non-RGD disintegrin from C. d. collilineatus venom. a Tris-tricine-SDS-PAGE (16.5%), in non-reduced conditions and the gel was stained with 0.2% Coomassie Brilliant Blue G-250. MW1 – molecular mass (97.0–14.4 kDa); Cdc – C. d. collilineatus venom; 2 – Fraction 2 from RP-FPLC first step; 6–8 – fractions 6, 7 and 8, respectively, from RP-FPLC second step; MW2 – molecular mass (26.6–1.06 kDa). b Mass spectrum of Fraction 6 eluted from RP-FPLC second step obtained by MALDI-TOF (positive linear mode) using α-cyano-4-hydroxycinnamic acid (α-CHCA) matrix