Table 1.
Primer sequences used for plasmids construction
| Name | Primer sequence (5′-3′) | Vector |
|---|---|---|
| shTfR1 | shTfR1:ggacatgctcatctaggaaca | pLVX-shRNA1 |
| shCtrl: gcttgcacttaaagtagtaga | ||
| TfR1 | F: ctcaagcttcgaattcatgatggatcaagctagat | pLVX-DsRed |
| R: ggcgaccggtggatcccgttaaaattcattgtcaat | ||
| TfR1-Out | F: tgatatcggatccgaattcgcctattgtaaacgtgtag | pET-32a-c(+) |
| R: gcaagcttgtcgacaaattcattgtcaatgtc | ||
| F: cttaaggcctctgtcgacgcctattgtaaacgtgtag | pAcGFP1-C | |
| R: ccggtggatccgccagaattcttaaaattcattgtcaatgt | ||
| TGEV-S1 | F: tctagcccgggcggatcctgtgctagttatgtggct | pCMV-C-HA |
| R: atcgtatgggtatctagaatttgtataattatatatagag |