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. 2018 Oct 20;19:761. doi: 10.1186/s12864-018-5134-7

Fig. 1.

Fig. 1

Experimental design. All plants were first germinated and grown at 24 °C /14 °C day/ night temperature. The cold treated plants were exposed to 14 °C / 2 °C day/night temperatures for three days starting on day 13 after planting and then returned to 24 °C /14 °C. Leaf samples for RNA extraction, depicted by the letter S, were taken 24 h into the cold exposure (D1) and 24 h after cold exposure (D4). Two control groups of plants were planted with one and three day delays, so the developmental stages of the cold and control treated plants were the same at sampling time