Table 1.

Properties of mammalian viral siRNAs (vsiRNAs) compared to fruit fly vsiRNAs

Virus (strain/mutant)	Virus family	Viral genome	Host	Experimental system	vsiRNAs*				Ref
					%of total mapped reads	%of total mapped miRNAs	%of total viral small RNA reads	strand ratio (+/−)
Nodamur a virus (NoVΔB2 )	Nodaviridae	Segmented positive-strand RNA	Hamster	Cultured BHK21 cells	0.06%	0.13%	40%	2.2	9
			Mice	Embryonic stem cells	0.02%	0.04%	44%	1.8	10
				Newborn mice	0.24%	0.3%	85.4%	0.96	9
Encephalomyocarditis virus (2887A/91)	Picornaviridae	Positive-strand RNA	Mice	Embryonic stem cells	0.19%	0.3%	33%	3.1	10
Human enterovirus 71 (HEV71D23A)	Picornaviridae	Positive-strand RNA	Human	Cultured 293 cells	0.04%	2.4%	32%	1.5	12
Influenza A Virus (PR8/del NS1)	Orthomyxoviridae	Segmented negative-strand RNA	Human	Cultured A549 cells	0.59%	2.4%	54%	1.04	11
Flock house virus (FHVΔB2)	Nodaviridae	Segmented positive-strand RNA	Fruit fly	Adult flies	0.43%	1.9%	97.4%	0.74	13

^*The sRNA-seq libraries in refs 9, 11, 12 and 13 were re-calculated using the following set of definitions. Total vsiRNAs include only virus reads of 21 to 23 nt in length for mammalian viruses or 20 to 22 nt in length for the fly virus. Total viral small RNA reads include those in the size range of 18 to 28 nt except the small RNAs of EMC V and NoVAB2 from mESCs, which are 19 to 44 nt in length. Total mapped reads in a given sRNA-seq library include all validated reads mapped to both the host and viral genomes.