Skip to main content
. Author manuscript; available in PMC: 2019 Oct 15.
Published in final edited form as: J Comp Neurol. 2018 Oct 15;526(15):2493–2508. doi: 10.1002/cne.24508

Table 3: Insertion mapping of donor DNA.

Sequencing of CRISPR lines showed the integration of the donor plasmid (bolded) into genomic DNA (standard), including the presence of insertions (italicized) in two of the three lines.

Line Sequence Cut Site
Relative to
Target (bp)		 Insertion
Length
(bp)
net: mCFP ...acacgcctggggcc... −7 0
net: mTdTomato ... acacgcctgcggtgcaggtggggcc... −6 10
net: H2B-GCamp6f ... atcccattccagggaggttccaggtggggcc... −24 16