Table 3.
Effects of NOVO, CAP, and the TRPV1 inhibitors on cell membrane integrity of IEC-6/TRPV1+ cells (percentage of LDH release was used as an index).
| Drugs | Conc.(μM) | LDH release (gprot⋅mL -1) |
|||
|---|---|---|---|---|---|
| 12 h |
24 h |
||||
| IEC-6/TRPV1 + | IEC-6/TRPV1- | IEC-6/TRPV1 + | IEC-6/TRPV1- | ||
| Control | 0.593 ± 0.086 | 0.668 ± 0.071 | 0.487 ± 0.073 | 0.572 ± 0.063 | |
| RR | 10 | 0.406 ± 0.056 | 0.552 ± 0.039 | 0.301 ± 0.061 | 0.501 ± 0.094 |
| SB-705498 | 0.1 | 0.469 ± 0.124 | 0.509 ± 0.155 | 0.423 ± 0.149 | 0.537 ± 0.019 |
| CAP | 25 | 0.380 ± 0.165 | 0.680 ± 0.205 | 0.311 ± 0.051 | 0.462 ± 0.101 |
| NOVO | 1 | 0.415 ± 0.169 | 0.638 ± 0.131 | 0.353 ± 0.026 | 0.433 ± 0.127 |
| 5 | 0.356 ± 0.090 | 0.651 ± 0.120 | 0.300 ± 0.052 | 0.554 ± 0.103 | |
| 10 | 0.337 ± 0.005 | 0.535 ± 0.119 | 0.299 ± 0.072 | 0.502 ± 0.106 | |
Cell cytotoxicity was determined by a LDH release assay kit as described in Section “Materials and Methods.” All data are the mean ± SD values of the ratio of release of LDH into cell culture supernatants to intracellular fluid. Cells treated with 1% ethyl alcohol in 10% culture medium were considered as the control group. LDH activity (U⋅mL-1) = [(OD treat – OD control)/(OD standard – OD blank)] × 0.2 × N. LDH activity of Suspension (U⋅gprot-1s) = LDH activity/protein concentration; N, dilution ratio; 0.2, the concentration of standard solution. LDH release % (gprot⋅mL-1) = LDH activity of supernatants/LDH activity of Suspension.