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. 2018 Oct 15;9:1461. doi: 10.3389/fphys.2018.01461

FIGURE 1.

FIGURE 1

Effects of femoral artery occlusion on TNF-α signal in sensory nerves. (A) Seventy-two hours of femoral artery occlusion increased the levels of TNF-α in the DRG tissues as compared with them in DRG of control limbs (n = 6 in each group); and PTX given into the hindlimb muscles attenuated amplification of TNF-α in the DRG tissues of limbs with femoral artery occlusion (n = 8). ∗P < 0.05, occlusion group vs. control and occlusion with prior PTX. (B) Immunofluorescence was used to examine double-labeling for TNF-α and peripherin/NF-200 (n = 3). Peripherin was used to label DRG neurons that project thin C-fibers. NF200 was used to identify A-fibers of DRG neurons. Representative photomicrographs show co-existence of TNF-α and peripherin staining in DRG neurons (top panel), whereas few TNF-α and NF-200 staining were observed in DRG neurons (bottom panel). Arrows indicate representative positive cells for both TNF-α and peripherin after they were merged. Scale bar = 50 μm. (C) Representative bands (left panel) and averaged data (right panel), demonstrating that femoral artery occlusion upregulated protein expression TNF-α receptor subtype TNFR1, but not TNFR2. A significant difference in TNFR1 was seen between control and occluded groups. ∗P < 0.05 vs. control. n = 6 in each group.