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. 2018 Oct 15;9:2360. doi: 10.3389/fimmu.2018.02360

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Copyright © 2018 Pesce, Squillario, Greppi, Loiacono, Moretta, Moretta, Sivori, Castagnola, Barla, Candiani and Marcenaro.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CD56^bright and CD56^dim NK cell subsets isolation strategy. (A) Sorting separation strategy for CD56^bright/CD16⁻ and CD56^dim/CD16⁺ NK cells subsets. (B) Informative NK cell surface markers pre- and post-sorting are shown (one representative donor). (C) Post-sorting statistical analysis of informative NK cells surface markers. Data represents the mean ± SD, n = 10 donors. T-test analysis comparing CD56^bright/CD16⁻ and CD56^dim/CD16⁺ NK cell subsets indicated as ****p < 0.0001. Color legend: Pre-sorting NK cells are indicated in black, CD56^bright/CD16⁻ NK cells are indicated in light blue and CD56^dim/CD16⁺ NK cells are indicated in purple.