Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Sep 24;201(9):2683–2699. doi: 10.4049/jimmunol.1701783

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2018 The Authors

This article is distributed under the terms of the CC BY 4.0 Unported license.

PMC Copyright notice

FIGURE 3. — Analysis of whole blood. (A) Whole EDTA blood from adult rats was used to analyze forward versus side scatter (FSC/SSC) profiles by flow cytometry. SIRPα⁺ monocytes are colored red. (B) The percentages of PBMCs, granulocytes, and erythroblasts were determined by FSC/SSC profiles (n = 5 per genotype). (C) Cells were gated on SIRPα⁺ PBMC to determine the percentage of monocytes (n = 5 per genotype). (D) SIRPα expression was analyzed in whole EDTA blood gating on total live cells and live PBMC. Solid arrow highlights SIRPα^low granulocytes. Dotted arrow highlights SIRPα^high monocytes (n = 5 per genotype). (E) Whole EDTA blood was analyzed on an automated counter to determine the percentage of neutrophils and lymphocytes in WBCs (n = 6^+/+, 10^+/−, and 8^−/−). Graphs show the mean + SEM. Significance compared with wild type is indicated by *p = 0.046 (erythroblasts) and 0.015 (monocytes), ***p < 0.0003, and ****p < 0.0001 using a t test.