Table II. Immunohistochemistry conditions.
| Target (Clone) | Supplier | Ag Retrieval | Dilution | Detection |
|---|---|---|---|---|
| CD68 (ED1) | Bio-Rad | Proteinase K 20 min, RT | 1:500 | ImmPRESS anti-mouse 15 min |
| 45 min | DAB, hematoxylin counterstain | |||
| CD163 (ED2) | Thermo Fisher Scientific | Proteinase K 20 min, RT | 1:100 | ImmPRESS anti-mouse 10 min |
| 60 min | DAB, hematoxylin counterstain | |||
| IBA1 rabbit polyclonal | Wako Pure Chemical Corp. | 0.01 M citrate buffer, pH 6.0 | 1:500 | EnVision anti-rabbit 40 min |
| 110°C 5 min | 30 min | DAB, hematoxylin counterstain | ||
| MRC1 rabbit polyclonal | Abcam | 1 mM EDTA, 0.1% Tween 20, pH 8 | 1:1000 | EnVision anti-rabbit 40 min |
| 16 h 60°C | 60 min | Vector NovaRED, hematoxylin counterstain | ||
| SIGLEC1 (ED3) | Bio-Rad | 0.01 M citrate buffer, pH 6 | 1:200 | ImmPRESS anti-mouse 30 min |
| 110°C 20 min | 2 h | Vector VIP, no counterstain. |
DAB, 3,3-diaminobenzidine.