Figure 3.

DON transformation ability of heterologously expressed DepA.

A. PQQ is essential for DepA activity. Reactions contained 100 μg ml⁻¹ DON, in Tris pH 7.5 with 15 μg of purified DepA in the presence or absence of 100 μM PQQ.

B. DepA completely transforms DON to 3‐keto‐DON. Each reaction consisted of 100 μg ml⁻¹ DON, 40 μM phenazine and 100 μM PQQ with or without 5 μg of DepA. Reactions were incubated overnight at room temperature and replicated with an independent purification.