Figure 1.

Mycelia growth, conidial morphology and yield of wild‐type, complemented and ▵MrMsn2 mutants strains on SMAY media and RT–qPCR for MrMsn2 expression during conidiation.

A. Transcription of MrMsn2 during conidiation on different cultivation days. 3 μl of conidial suspensions (1 × 10⁷ conidia ml⁻¹) was spotted on SMAY plates and cultured under continuous light at 25°C for 8 days. Stages of conidial development: inoculated conidia at initial culture time (day 0), blastospores (day 2), hyphal period (day 4), conidiation initiation (day 6) and conidia at start of maturation (day 8).

B. Images of colonies on SMAY plates for 3, 6, 9 and 12 days. 3 μl of conidial suspensions (1 × 10⁷ conidia ml⁻¹) was spotted on SMAY plates and cultured under continuous light at 25°C for 12 days. Bar = 5 mm.

C. Conidial yield of tested strains on SMAY medium after 6, 9 and 12 days of incubation. Error bars are standard error. * P < 0.05, ** P < 0.01, significantly different compared to wild type or 0 day incubation.