Fig. 4.

dBP4 inhibits IGF1-induced angiogenesis in vivo. 12 week old female BALB/c mice (n = 5/group) were injected subcutaneously with Matrigel®. After 7 days implants were excised and stained for CD31 (pointed arrow indicates CD31+ cells). Representative images of a PBS negative control, b VEGF positive control, c IGF1, d IGF1 and dBP4, e dBP4. f shows negative isotype control (scale bars 200 μm). g Mean ± SEM CD31+ cells in three fields of view/implant (n = 5). (*P < 0.05), **P < 0.01, ***P < 0.001) one-way ANOVA with Tukey post hoc test)