Figure 5.

Z-average size, polydispersity index (PDI), and ζ potential of liposomes at increased storage temperatures. First measurement (Ø PSD) was done with symmetrical liposomes of 80 mol % PC and 20 mol % PS. Then, liposomes were incubated with PSD at 28°C for 120 min and then DLS and ζ measured again (+PSD). Then, the PSD was removed via Ni-NTA agarose overnight at 20°C (measurement 14h). Aliquots of this sample were then stored at 20°C, 40°C, or 65°C and measured at the time points indicated. (A) Size and PDI of the samples were determined by DLS measurements. (B) ζ potential of the samples was determined. 96-h measurements at 40°C and 65°C are encircled to indicate that they refer to poor quality samples with large particle size and PDI. Error bars indicate three measurements of the same sample. To see this figure in color, go online.