Fig. 5.

Virus containing pentamer enters PDGFRα-KO HFF cells using an alternative route(s). HFFs, PDGFRα-KO cell clones (1-10 and 10-4), and CN-9 control cells were infected with viruses produced from fibroblasts or epithelial cells at a multiplicity of 3 FFU per cell. At 2 dpi, GFP expression from viral genomes was measured by flow cytometry to assay the percentage of infected cells, except for Merlin-GFP-epi and Merlin-GFP-fibro, where IE1 expression was measured to assay percentage of infected cells due to the dim GFP signal. The suffixes -epi and -fibro designate virus stocks produced in ARPE-19 epithelial and HFF fibroblast cells, respectively.