Fig. 2.

RKIP deficiency promotes IgE−FcεRI-mediated cytokine and chemokine production in the BMMCs. (A) WT and RKIP KO BMMCs were presensitized with 1 μg/mL of anti–DNP-IgE overnight and then activated with 100 ng/mL DNP-BSA. The expressions of the cytokines and chemokines were measured by real-time PCR. (B) The production of IL-6, TNF-α, IL-4, IL-5, IL-13, and GM-CSF was measured by ELISA at 24 h. (C) The BMMCs were transfected with negative control siRNA (siNC) or RKIP-specific siRNA (siRKIP) and treated as described in A. The expression of cytokines and chemokines was measured by real-time PCR. *P < 0.05; **P < 0.01; ***P < 0.001. Data are representative of three experiments. Data represent the mean and SD.