Fig. 6.

RKIP deficiency increases FcεRI-mediated PI3K/Akt/NF-κB axis signaling activation in mast cells. (A) Immunoblot analysis in lysates of the WT and RKIP KO BMMCs stimulated by IgE−FcεRI-mediated activation for the indicated times. (B–D) WT and RKIP KO BMMCs were presensitized with 1 μg/mL of anti–DNP-IgE overnight, pretreated with wortmannin (PI3K inhibitor) for 2 h, and then stimulated with 100 ng/mL of DNP-BSA for the indicated times. (B) Immunoblot analysis was performed to identify the total or phosphorylated proteins in lysates using indicated antibodies. (C) The β-hexosaminidase was measured as described in Materials and Methods. (D) The mRNA expression levels of cytokines and chemokines were measured by real-time PCR. ns, no significant difference; *P < 0.05; **P < 0.01; ***P < 0.001. Data are representative of three experiments. Data represent mean and SD.