Fig. 1.

Segmental labeling of hnRNPA2-LC polymers. (A) Wild-type amino acid sequence of the LC domain of hnRNPA2, residues 181–314. Isotopic labeling for solid-state NMR measurements on segmentally labeled hnRNPA2-LC polymers was restricted to the C-terminal segment shown in red. Residues with assigned solid-state NMR signals are underlined. Residues with β-strand backbone torsion angles determined by the experiments in this paper have an asterisk above them. (B) Schematic of the segmental labeling scheme. The N-terminal hnRNPA2-LC segment has 6x His and mCherry tags. The Gly-to-Cys mutation at residue 280 is expressed with the C-terminal segment. (C) TEM images of segmentally ¹³C,¹⁵N-labeled mCherry-hnRNPA2-LC polymers, negatively stained with uranyl acetate.