Fig. 2.

Nb-induced ST2⁻ and ST2⁺ mTh cells exhibit distinct gene expression profiles. (A) Experimental protocols for the infection of wild-type mice with Nb. ^#Assay: RNA sequencing. (B) The expression of the genes with a difference in expression of more than fivefold in ST2⁺ mTh cells compared with the other two groups obtained by RNA sequence analysis. (C and D) MA plots comparing the different expression of transcription factors (C) as well as cytokines and chemokines (D) between ST2⁺ and ST2⁻ mTh cells. Horizontal line denotes log₂[(ST2⁺ mTh)/(ST2⁻ mTh)], while vertical lines denote (1/2) × log₂[(ST2⁻ mTh) × (ST2⁺ mTh)]. (E) A qRT-PCR analysis of the indicated genes in ST2⁺ and ST2⁻ mTh cells. The minimum data values were set as one. The mean values (four data per group) are shown with the SEM. n.s., not significant. **P < 0.01; ***P < 0.001. (F) MA plots comparing the differential expression of surface markers between ST2⁺ and ST2⁻ mTh cells. (G) Staining profiles of indicated markers on ST2⁺ and ST2⁻ mTh cells are shown with the mean fluorescence intensity of cells in histograms. At least two independent experiments were performed and showed similar results. The mean of FPKM was used for analyses in B–D and F. We set positive/negative gates using staining with isotype controls in G.