Fig. 5.

Helminth-induced ST2⁺ mTh2 cells accumulated MBP-expressing eosinophils in the lungs, reducing the fecundity of Nb. (A) The expressions of the genes with a difference in expression of more than fivefold in the sorted eosinophils obtained from the lungs of ST2⁺ mTh cell-transferred mice compared with the other two groups according to an RNA sequence analysis were selected as depicted in SI Appendix, Fig. S7. The genes listed in two independent experiments are shown. (B) A qRT-PCR analysis of Prg2 and Epx in the sorted eosinophils obtained from the lung of naïve CD4⁺ T cell-, ST2⁻ mTh cell-, or ST2⁺ mTh cell-transferred mice. The minimum data values were set as one. The mean values (six data per group) are shown with the SEM. (C) Experimental protocols for the infection of the mice with Nb. ^#Assay: count numbers of eggs and worms. (D) Mice were killed 6 d after infection with Nb, as described in C. The absolute numbers of parasite eggs in feces per gram and the numbers of worms are shown. The mean values (six mice per group) are shown with the SEM. At least two independent experiments were performed and showed similar results. n.s., not significant. **P < 0.01; ***P < 0.001.