Fig. 4.

Inhibition of CFL, but not CIL, during cell contact-mediated symmetry breaking by the Dvl inhibitor NSC 668036. (A) Phase-contrast images show a spreading NRK-52E cell from the second round of seeding (red), making contact with and migrating away from the head of an approaching cell (green) in the presence of 50 µM NSC 668036. (B) Phase-contrast images of a spreading cell from the second round of seeding (red) making contact with and failing to follow the tail of a retreating cell (green) in the presence of 50 µM NSC 668036. White arrows indicate the direction of migration of the cell from the first round of seeding. Elapsed times are shown to the right. (Scale bars, 30 µm.)