Table 1.
Setting | Approaching cell maintaining original polarity, % | Stalled cell retreating from the approaching neighbor, % |
X intersection | 90.3 ± 3.1 (n = 93) | 88.2 ± 3.4 (n = 93)** |
T intersection | 82.3 ± 4.9 (n = 62) | 88.7 ± 4.1 (n = 62)*** |
Double seeding | 69.4 ± 3.7 (n = 160) | 73.1 ± 3.5 (n = 160)*** |
In all three settings tested (left column), a migratory cell approaches a stalled or spreading cell to make contact. The majority of stalled or spreading cells migrate away from the approaching cell following the same direction (right column), while the majority of approaching cells continue to migrate along the original direction (middle column). Significance was determined using a one-sample binomial test against the null hypothesis of equal probability of entering any branch. **P < 0.005, ***P < 0.0005.