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. 2018 Sep 28;115(42):E9962–E9970. doi: 10.1073/pnas.1812847115

Fig. 3.

Fig. 3.

Effect of ROS1 mutation on DOGL4 promoter DNA methylation in endosperms and embryos. (A) Bisulfite sequencing of −1.0-kb region of DOGL4 promoter in WT and ros1 mutants. Twelve-day-old seedlings of Col, ros1-4, ros1-6, C24, and ros1-1 were used for DNA isolation and bisulfite sequencing. DNA methylation at CG and CHG contexts was increased in all ros1 mutants. (B–F) DNA methylation analysis of −1.0-kb region (Fragment 3) (B and D) and −0.5-kb region (Fragment 1) (C–E) of DOGL4 promoter. (B and C) DNA methylation analysis in F1 endosperms from reciprocal crosses between ros1-1 and ros1-4 (B) and in ros1-4 endosperms (C). (D–F) DNA methylation analysis in F1 embryos from reciprocal crosses between ros1-1 and ros1-4 (D), ros1-4 embryos (E), and Col embryos (F).