Table 2.

Protein production from multiple- and single-round translation reporters in rpoB mutant strains

Operon	Reporter design	rpoB(H526Y)*		rpoB(I572N)†
		β-Gal activity‡	Product ratio§	β-Gal activity‡	Product ratio§
sspAB	Multiple-round	347 ± 55	3.7:1	408 ± 50	2.9:1
		95 ± 5.7		143 ± 7.3
	Single-round	12 ± 0.2	4.4:1	7.5 ± 0.8	1:1.1
		2.7 ± 0.3		8.4 ± 0.1
purMN	Multiple-round	49 ± 0.4	2.6:1	55 ± 1.6	2:1
		19 ± 0.4		28 ± 0.8
	Single-round	1.1 ± 0.1	3.7:1	1.2 ± 0.1	2.4:1
		0.3 ± 0.1		0.5 ± 0.1
moeAB	Multiple-round	47 ± 1.7	3.1:1	87 ± 0.7	2.6:1
		15 ± 0.4		33 ± 2.1
	Single-round	1.9 ± 0.1	6.3:1	3.2 ± 0.1	4:1
		0.3 ± 0.1		0.8 ± 0.1

^*Causes increased transcription elongation rate.

^†Causes decreased transcription elongation rate.

^‡β-Gal activity based on cleavage of CPRG. Data represent mean ± SEM (n ≥ 3). Background ≤0.1, based on measurements of CSH142 cells.

^§First gene:second gene.