Fig. 1.
Resource partitioning between Brassica rapa seedling organs after 9 h of light treatment. Five-day-old B. rapa seedlings grown in long days were subjected to high or low R:FR at ZT2. (A) Hypocotyl growth was measured from time-lapse images with 30-min intervals. The black bar in the x axis represents the dark period. Data represent means ± 2 SE; n = 9. (B) Hypocotyl biomass after 9 h of light treatment. Data are represented as standard boxplots representing median and interquartile (IQR) range between the 25th and 75th percentiles. Whiskers extend to 1.5-fold the IQR; n = 20. (C) Incorporation of radiolabeled C in intact seedlings or seedlings from which cotyledons had been removed (decapitated), harvested immediately after a 10-min pulse of 14CO2 following 9 h of light treatment. Values are expressed as the percentage of label recovered in each sample compared with the average total label of high-R:FR–treated intact plants. Data represent means ± 2 SE; n = 4. (D) 14C incorporation in cotyledon, hypocotyl, and root tissue after a 1-h chase. Values are expressed as a percentage of total label recovered in each organ. Data represent means ± 2 SE; n = 4. Asterisks indicate significant difference. Significance codes: *0.05 > P > 0.01, **0.01 > P > 0.001, ***P < 0.001.