Fig. 3.

Metabolite levels in Brassica rapa hypocotyls during the first 24 h of the shade avoidance response. Five-day-old B. rapa seedlings were grown in long days and subjected to high or low R:FR at ZT2 (t = 0). Hypocotyls were analyzed for glucose (A), fructose (B), sucrose (C), and starch (D) levels at the indicated time points after the start of low R:FR, including just before dark and immediately after dawn. The black bar in the x axis represents the dark period. Data represent means ± 2 SE; n = 4 replicates of four pooled seedlings. Corresponding cotyledon data are shown in SI Appendix, Fig. S3.