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. 2018 Oct 1;115(42):E9822–E9831. doi: 10.1073/pnas.1808243115

Fig. 9.

Fig. 9.

Rescue of the Aa5HT2B-depletion phenotypes by CRISPR-Cas9 genomic disruption of ILP5. (A) Comparison of female body size (Left) and wing length (Right) between WT control and the Δ(2B+ilp5) mutants (compound knockout of Aa5HT2B and ilp5 genes by CRISPR-Cas9). [Scale bars: 2 mm (Left) and 1 mm (Right).] All females (n = 285) were used for measurement. *P < 0.05; ***P < 0.001. (B and C) Rescue of pupation (B) and eclosion (C) rates in double CRISPR-Cas9 depletion of Aa5HT2B and ilp5. The log-rank test rests for the pupation curves and eclosion curves (times-to-event) are P < 0.0001; thus, the curves are significantly different; 200 individuals in one culture container were used for counting.