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. 2018 Oct 1;115(42):E9822–E9831. doi: 10.1073/pnas.1808243115

Fig. 10.

Fig. 10.

Recovery of the nutrition status in double CRISPR-Cas9 depletion of Aa5HT2B and ilp5. (A) Lipid droplets in the fat-body dissected from adult females (24-h PBM) and larvae (fifth day after egg hatching) of WT and mutants were detected by Nile red and visualized under a confocal microscope (Scale bars, 25 μm.). Blue, DAPI staining. (B and C) The changes in TAG and glycogen levels in Aa5HT2B-ilp5 CRISPR-Cas9 mutant adults (B) and fourth-instar larvae (C) were alleviated. Data represent three biological replicates (six individuals in each replication) with three technical replicates and are shown as mean ± SEM. **P < 0.01; ***P < 0.001.