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. 2018 Sep 27;115(42):10556–10563. doi: 10.1073/pnas.1811100115

Fig. 2.

Fig. 2.

Heterophilic trans interactions of ASTN1 and CDH2. Drosophila S2 cell-adhesion assays were prepared in four conditions: Cdh2;GFP + mCherry;GFP (A), Cdh2;GFP + Astn1;mCherry (B), Cdh2;GFP + Astn1;mCherry + ASTN1 Fab (C), and Cdh2-Δ390;GFP + Astn1;mCherry (D). ASTN1-positive cells were adhering to the CDH2-expressing aggregates after 30 min (arrows in B1), with more coaggregation seen after 1 h (arrows in B2) and 2 h (arrows in B3), indicating heterophilic trans interactions. Significantly lower proportions of cells were adhering to the aggregates in the conditions with cells expressing control vector (A) or Astn1;mCherry blocked with ASTN1 Fab fragments (C). (D) Expression of CDH2-Δ390;GFP did not result in cell aggregation within 2 h, demonstrating the importance of the cadherin ectodomain for homophilic and heterophilic interactions and cell adhesion. The proportion of mCherry-expressing cells in the CDH2;GFP-positive aggregates is quantified in E. **P < 0.01; ***P < 0.001. (Scale bars: 50 µm in A–D and 10 µm in Inset in B3.)