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. 2018 Oct 1;115(42):10768–10773. doi: 10.1073/pnas.1811919115

Fig. 5.

Fig. 5.

Modeling the effect of FtsZL272E on FtsZ polymer length and the effect of increasing FtsZ and decreasing GTPase activity on suppressing FtsZL272E toxicity. (A) Treadmilling model for FtsZ was used to assess the effect of FtsZL272E on FtsZ filament length with FtsZ polymer-length distribution as a function of the ratio of FtsZL272E to FtsZ. The fourth panel plots the average polymer length versus the FtsZL272E-to-FtsZ ratio. (B) Increasing FtsZ and reducing GTPase activity suppress the toxicity of FtsZL272E. JS238/pSD334-L272E (pEXT22, Ptac::ftsZL272E) containing plasmids expressing ftsZ (pBEF0), ftsZD212G (pBEF2), or a vector control (pGB2) was subjected to a spot test on plates with increasing IPTG.