Fig. 3.

Amino acid alignment of Populus trichocarpa nitrilases, PtNIT1, PtNIT2, PtNIT3, and Potri.009G161000 with SbNIT4A from Sorghum bicolor and AtNIT1/4 from Arabidopsis. Black boxes mark conserved residues and grey boxes mark residues with similar physicochemical properties. Residues forming the catalytic triad are highlighted with arrows. Sequence motifs that are conserved throughout the nitrilase family are marked by black lines. The TCP domains of PtNIT2 and PtNIT3 are underlined and written in italics