Table 1.
Activity of PtNIT1 with different aromatic and aliphatic nitriles as substrates. The purified recombinant protein was incubated with the potential nitrile substrate and the formation of the corresponding acids was analyzed using LC-MS/MS
| substrate | substrate structure | activity |
|---|---|---|
| β-cyanoalanine |
|
yes |
| 3-phenylpropionitrile |
|
yes |
| benzyl cyanide |
|
yes |
| 4-hydroxybenzyl cyanide |
|
yes |
| indole-3-acetonitrile |
|
yes |
| isovaleronitrile |
|
no |
| 3-butenenitrile |
|
no |
| butyronitrile |
|
no |