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PD-L1 expression is reduced in CT26 cells by lenti-viral expression of PD-L1 shRNA. (A) Representative blot of PD-L1 protein expression levels. (B) mRNA expression of PD-L1 was significantly reduced after transfection with shRNA PD-L1. (C) Protein expression of PD-L1 was significantly reduced after transfection with shRNA PD-L1. *P<0.05 vs. control. (One-way analysis of variance with Newman-Keuls). Sh, short hairpin; PD-L1, programmed cell death ligand 1.
