Table I.
Primer sequences for the target genes.
| Genes | Primer sequences (5′-3′) | Primer length (bp) | Product length (bp) | Annealing temperature (°C) |
|---|---|---|---|---|
| PD-L1 | 111 | 57 | ||
| F | CCATCTTATTATGCCTTGGTGTAG | 24 | ||
| R | TTTGCTTCTTTGAGTTTGTATCTTG | 25 | ||
| PI3K | 140 | 54 | ||
| F | CCAACACCTTCATCATCC | 18 | ||
| R | CTCCTCCTCCTGCTTCTT | 18 | ||
| AKT | 231 | 58.7 | ||
| F | TGTGATCTTAATGTGCCCGTC | 21 | ||
| R | TACTCTTTCCAGACCCACGAC | 21 | ||
| GAPDH | 100 | 58.6 | ||
| F | GAAGGTCGGAGTCAACGGAT | 20 | ||
| R | CCTGGAAGATGGTGATGGG | 19 |
PD-L1, programmed cell death ligand 1; AKT1, RAC-α serine/threonine-protein kinase 1; PI3K, phosphatidylinositol 3-kinase; F, forward; R, reverse.