Figure 5.

IFITM3 is the targeting gene of miR-29a. (A and B) Western blot analysis screened 14 candidate miRNAs and indicated that miR-29a could markedly suppress the protein expression of IFITM3 in HCCLM3 cells. (C) The 3′-UTR of IFITM3 contained a binding site for miR-29a. (D) Dual-Luciferase reporter gene assay revealed that miR-29a mimics could reduce the luciferase activities of IFITM3-WT reporter vector rather than IFITM3-MUT, indicating that IFITM3 was the targeting gene of miR-29a. **P<0.01. N.S., non-significant; WT, wild-type; MUT, mutant type; IFITM3, interferon-induced transmembrane protein 3.