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. 2018 Sep 18;40(6):3144–3154. doi: 10.3892/or.2018.6713

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Copyright: © Dong et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — Inhibition of pancreatic CSC-like subpopulations by Rau. (A) Cell proliferation of unsorted cells, DCV⁺ cells (non-CSC-like) and DCV⁻ cells (CSC-like) with Rau treatment for 48 h (mean ± standard deviation of three independent experiments). (B) Representative images of the MIA PaCa-2 spheroids from unsorted cells, DCV⁺ cells and DCV⁻ cells with and without Rau treatment (magnification, ×10). The number and size of MIA PaCa-2 spheroids are shown in bar graph. *P<0.05; **P<0.01 and ***P<0.001. CSC, cancer stem cell; Rau, Rauwolfia vomitoria; DCV, Dye Cycle Violet; IC₅₀, 50% inhibitory concentration.