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Binding affinities of SULT1B1-WT and L145V for PAP and PAPS. The SULT1B1-WT (opaque shapes) and L145V (hollow shapes) dissociation constants for PAPS (squares) and PAP (circles) were measured by intrinsic fluorescence. Both isoforms exhibit a similar Kd for PAPS (~900 nM), while PAP binds less potently to the L145V isoform (405 nM as opposed to 106 nM).
